Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1974 Jun;61(3):780-8.
doi: 10.1083/jcb.61.3.780.

Isopycnic centrifugation of chromatin in renografin solutions

R T Chan, I E Scheffler

Isopycnic centrifugation of chromatin in renografin solutions

R T Chan et al. J Cell Biol. 1974 Jun.

Abstract

Solutions of Renografin (30-60%) can be centrifuged to form density gradients in the range from 1.0 g/cm(3) to 1.4 g/cm(3) or, alternatively, preformed gradients can be made which under appropriate conditions of centrifugation have an indefinite stability. Such solutions have a low viscosity and a relatively low ionic strength. The density of DNA in such solutions is surprisingly low ( approximately 1.14 g/cm(3)). Crude chromatin can be sedimented to an equilibrium position in such gradients, corresponding to a density of 1.2(4) g/cm(3), or slightly lower, depending on the method of preparation. The complex is shown to contain DNA, RNA, protein, and possibly some lipoprotein. Most of the RNA can be removed with RNase without any significant effect on the density of the chromatin.

PubMed Disclaimer

References

    1. J Bacteriol. 1970 Nov;104(2):839-50 - PubMed
    1. Science. 1968 Jan 5;159(3810):47-56 - PubMed
    1. J Cell Physiol. 1973 Apr;81(2):199-216 - PubMed
    1. FEBS Lett. 1973 Jul 1;33(2):221-4 - PubMed
    1. Biochemistry. 1973 Jul 3;12(14):2766-73 - PubMed