Optic nerve gliomas. II. Cytological characteristics. Observations on cell and tissue cultures

Abstract

Cytological characteristics of optic nerve gliomas were studied parallel with histological structures in cell and tissue cultures. Histologically the tumours consisted of four different cell types: spindle-shaped elongated cells, round cells, stellate forms, and multinucleated cells. Dynamic properties of cultured optic glioma cells showed that stable cellular elements of the parent tissue arise from two fundamental populations, viz from elongated (piloid), and from round cells. Cells of both populations displayed a series of changes in the course of their cultures. A certain part of the slender elongated cells enlarged and became plump piloid cells of various shapes, or big stellate astrocytes. Giant multinucleated variants of these forms also emerged by fusion of the mononucleate forms. The round cells gradually turned into immature Astrocytes, and by a subsequent cell fusion they gave birth to big stellate cells. With the ageing of the cultures a polymorphous cellular picture developed which was mainly due to the active migratory and intracellular movement of the elongated and round cellular elements. Mitoses played a negligible role in this process. Several characteristics suggest that the round shapes are immature glia cells, and represent the least differentiated elements among all the cell components. The stellate and giant multinucleated forms might be considered as end-products of glia cell differentiation and they could originate both from the elongated and the round cells.