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Comparative Study
. 1979;20(2):270-5.
doi: 10.1186/BF03546618.

Plasma corticosteroid levels in laying hens. Effect of two different blood sampling techniques and of rough handling of the animals

B Eskeland, A K Blom
Comparative Study

Plasma corticosteroid levels in laying hens. Effect of two different blood sampling techniques and of rough handling of the animals

B Eskeland et al. Acta Vet Scand. 1979.

Abstract
in English, Norwegian

Corticosteroids were measured in blood samples collected from 10 hens in two series with a time interval of nine days. In the first series blood was collected by venipuncture (wing vein), in the second by cardiac puncture. In each series, sampling took place immediately before (control), and 5 min as well as 18 h after intentional rough handling. Only samples obtained by cardiac puncture 5 min after rough handling showed markedly elevated hormone levels.

Corticosteroids were also measured in blood collected from two other groups of hens, each including 10 birds. Samples were taken at 0 (control), 1, 5 and 40 min, one group being sampled by venipuncture, the other by cardiac puncture, None of the groups were subjected to rough handling. The hormone levels in samples obtained were significantly higher (P < 0.005) than the levels in the corresponding samples obtained by venipuncture.

Kortikosteroider ble malt i blodprøver fra 10 høner, tatt i to serier med ni dagers intervall. I den første serien ble blod tatt med vingevenepunktur, i den andre serien med hjertepunktur. I begge seriene ble blodprøvene tatt like før (kontroll), 5 min og 18 timer etter hardhendt behandling. Bare blodprøven som ble tatt med hjertepunktur 5 min etter hardhendt behandling viste markert forøket hormonnivå.

Kortikosteroidnivået ble målt i blod tatt fra to andre grupper av høner, hver gruppe inneholdt 10 dyr. Prøvene ble her tatt ved tid 0 (kontroll), 1, 5 og 40 min, hvorav den ene gruppen ble tappet med venepunktur og den andre med hjertepunktur. Ingen av gruppene her ble utsatt for hardhendt behandling. Prøver tatt 5 og 40 min etter kontroll med hjertepunktur viste signifikant (P > 0,005) høyere hormon-nivå enn tilsvarende prøver tatt med venepunktur.

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