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Comparative Study
. 1974 Jul;28(1):80-5.
doi: 10.1128/am.28.1.80-85.1974.

Comparison of rapid methods for analysis of bacterial fatty acids

C W Moss, M A Lambert, W H Merwin
Comparative Study

Comparison of rapid methods for analysis of bacterial fatty acids

C W Moss et al. Appl Microbiol. 1974 Jul.

Abstract

When rapid gas-liquid chromatography methods for determination of bacterial fatty acids were compared, results showed that saponification was required for total fatty acid analysis. Transesterification with boron-trihalide reagents (BF(3)-CH(3)OH, BCl(3)-CH(3)OH) caused extensive degradation of cyclopropane acids and was less effective than saponification in releasing cellular hydroxy fatty acids. Digestion of cells with tetramethylammonium hydroxide was unsatisfactory because of extraneous gas-liquid chromatography peaks and because of lower recovery of branched-chain and hydroxy fatty acids. A simple, rapid saponification procedure which can be used for total cellular fatty acid analysis of freshly grown cells is described.

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References

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