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. 1975;26(3):251-5.
doi: 10.1007/BF00281461.

Observations on specific giemsa staining of the Y and on selective oil destaining of the chromosomes

B Dallapiccola, N Ricci

Observations on specific giemsa staining of the Y and on selective oil destaining of the chromosomes

B Dallapiccola et al. Humangenetik. 1975.

Abstract

The human Y chromosome can be differentially stained with Giemsa using simple procedures. This phenomenon is strikingly to that observed with quinacrine fluorescence. The specific Giemsa-Y stain may be selectively removed by the action of an oil. The same oil, under certain conditions, selectively removes Giemsa stain from all chromosomes, resulting in R- and T-banding patterns. These bands, which are obtained through subtraction of dye from Giemsa-stained chromosomes, allow slides to be further processed.

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