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. 1974 Jun;139(3):709-14.
doi: 10.1042/bj1390709.

Iron and aconitase activity

O Gawron, A Waheed, A J Glaid 3rd, A Jaklitsch

Iron and aconitase activity

O Gawron et al. Biochem J. 1974 Jun.

Abstract

Aconitase activated with Fe(2+), cysteine and ascorbate incorporates 1 g-atom of Fe(2+)/mol. Loss of this Fe(2+) by transfer to ferrozine, a Fe(2+) chelator, results in loss of activity. Ascorbate increases the rate of transfer of the essential Fe(2+) whereas citrate retards the rate of transfer. Transfer of Fe(2+) from inactive aconitase, 2 g-atoms of Fe/mol, can be accomplished in the presence of urea and ascorbate. The correlation of activity with the presence of an added g-atom of Fe(2+)/mol leads to the conclusion that active aconitase has only one active site per mol.

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