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. 1967 Jul;7(4):353-64.
doi: 10.1016/S0006-3495(67)86593-7.

Cell growth and division. II. Experimental studies of cell volume distributions in mammalian suspension cultures

E C Anderson, D F Petersen

Cell growth and division. II. Experimental studies of cell volume distributions in mammalian suspension cultures

E C Anderson et al. Biophys J. 1967 Jul.

Abstract

Experimental proof is given that the volume distribution spectrum of mammalian cells in suspension culture can be determined accurately with a Coulter spectrometer. Stable spectra corresponding to the predictions of a mathematical model are observed under favorable conditions of growth. Cell volume spectrometry appears to be a useful method for diagnosing the state of the culture with respect to past uniformity of growth rate and present population age distribution. In addition, it offers a method for quantitative study of the laws governing cell growth and division.

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References

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