Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1967 Jul;15(4):826-9.
doi: 10.1128/am.15.4.826-829.1967.

Lipid patterns of selected microorganisms as determined by gas-liquid chromatography

J E Steinhauer, R L Flentge, R V Lechowich

Lipid patterns of selected microorganisms as determined by gas-liquid chromatography

J E Steinhauer et al. Appl Microbiol. 1967 Jul.

Abstract

The cellular lipid patterns of seven strains of microorganisms were examined by gas-liquid chromatography in this preliminary study. The chloroform methanol-soluble lipids were extracted by the Soxhlet method from dried cultures which had been grown at 25 +/- 2 C for 18 hr with mechanical shaking. The cellular extract was methylated by use of a low temperature sulfuric acid method, and the resulting methyl esters were chromatographed. Considerable differences in the lipid patterns among the seven microorganisms tested indicated that this method might be useful for the identification of closely related microbial genera, and possibly for species differentiation.

PubMed Disclaimer

References

    1. Science. 1966 Mar 25;151(3717):1543-4 - PubMed
    1. Nature. 1965 Jun 19;206(990):1272-3 - PubMed
    1. Appl Microbiol. 1966 Jul;14(4):513-24 - PubMed
    1. J Dairy Sci. 1966 Jun;49(6):624-7 - PubMed
    1. J Bacteriol. 1963 May;85:1039-44 - PubMed

LinkOut - more resources