Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 May 1;179(2):425-30.
doi: 10.1042/bj1790425.

The structure and amount of actin in IMR-90 fibroblasts

P J Anderson

The structure and amount of actin in IMR-90 fibroblasts

P J Anderson. Biochem J. .

Abstract

Double-labelling and peptide isolation have been used to examine the homology between the actin of IMR-90 human embryo fibroblasts and muscle actin. After separation of mixtures of [14C]carboxymethylated muscle actin and [3H]carboxymethylated proteins of IMR-90 cells of electrophoresis on sodium dodecyl sulphate-containing polyacrylamide gels, peptides were generated from the material co-migrating with actin by digestion with chymotrypsin. Peptides homologous with peptides accounting for Cys-217, Cys-256, Cys-284 and Cys-373 of muscle actin are present in this material, but no peptide homologous with a Cys-10-containing peptide was detected. From the amount of actin-derived peptides present, the actin content of IMR-90 fibroblasts was calculated to be 4.2% of the total protein of these cells.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Anal Biochem. 1964 Dec;9:401-10 - PubMed
    1. J Exp Med. 1954 Feb;99(2):167-82 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Jul;74(7):3019-22 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Oct;74(10):4491-5 - PubMed
    1. Proc Natl Acad Sci U S A. 1976 Nov;73(11):4065-9 - PubMed