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. 1979 Apr 15;180(1):37-44.
doi: 10.1042/bj1800037.

Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport

K M Brindle, F F Brown, I D Campbell, C Grathwohl, P W Kuchel

Application of spin-echo nuclear magnetic resonance to whole-cell systems. Membrane transport

K M Brindle et al. Biochem J. .

Abstract

A new method for studying membrane transport is presented. High resolution n.m.r. is used to measure the distribution of small molecules between the intracellular and extracellular compartments. The method uses spin-echo techniques and relies on a difference in the magnetic susceptibility of the media inside and outside of cells. It also provides simultaneous information on the metabolic status of the cell. The method is illustrated by a study of alanine and lactate transport in the human erythrocyte.

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