Mycoplasma: nutritional studies of laboratory strains

Abstract

Growth of 12 laboratory strains of Mycoplasma isolated from cattle, chicken, goat, man and sewage were studied on 20 different solid mediums and 9 liquid mediums. Growth on solid mediums was evaluated on the basis of appearance of visible colonies, while growth in liquid mediums was determined by light transmission technique. A solid medium of Bacto PPLO agar + Bacto PPLO serum fraction (1 %) + horse blood (5 %), and 0.005 % of 8 nucleosides (adenosine, cytidine, guanosine, deoxyadenosine, deoxycytidine, deoxyguanosine, thymidine, and uridine) was unsurpassed by any other medium. Studies of atmospheric requirements demonstrated that all strains grew in jars containing either air or nitrogen (100 %). One strain preferred an atmosphere with 10 % CO₂, while 3 strains were inhibited by this concentration of carbon dioxide.