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Comparative Study
. 1975 Feb 22;1(7904):426-8.
doi: 10.1016/s0140-6736(75)91492-0.

Microplate enzyme-linked immunosorbent assay for chagas' disease

A Voller, C Draper, D E Bidwell, A Bartlett
Comparative Study

Microplate enzyme-linked immunosorbent assay for chagas' disease

A Voller et al. Lancet. .

Abstract

A microscale immunosorbent assay (E.L.I.S.A.) for the measurement of antibody in Chagas' disease (American trypanosomiasis) is described. The test was assessed on plasma samples from people living in Bahia, Brazil. 98% of the individuals with positive Trypanosoma cruzi immunofluorescent-antibody (I.F.A.) tests gave E.L.I.S.A. reading considered as positive. Less than 2% of those with negative I.F.A. tests had positive E.L.I.S.A. reactions. This simple test may be suitable for use in large seroepidemiological surveys.

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