Somatic antigen 2 inheritance in Salmonella groups B and D

Abstract

Somatic (O) antigen 2 of Salmonella paratyphi A replaced somatic antigen 4 of an S. typhimurium recipient as the consequence of mating with an S. paratyphi A var. durazzo Hfr strain. The genetic determinants of these O antigens behaved in this cross as alleles of a common O locus, which is linked to the determinant of histidine biosynthesis, his. By employing phage lysates obtained by growth of P22 on an S. typhimurium hybrid which had received his and O-factor 2 determinants from the S. paratyphi A Hfr, it was possible to cotransduce the his and O-antigen 2 genes to both S. typhimurium and S. typhosa. S. typhimurium transductants which received somatic antigen 2 concurrently lost O-antigen 4, and S. typhosa transductants receiving O-antigen 2, lost their native O-antigen 9. These results indicate that the genetic determinants of O-antigens 2, 4, and 9 occupy the same O locus in S. paratyphi A, S. typhimurium, and S. typhosa, respectively, and are probably allelic.