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• Improving the production of 9α-hydroxy-4-androstene-3,17-dione from phytosterols by 3-ketosteroid-Δ¹-dehydrogenase deletions and multiple genetic modifications in Mycobacterium fortuitum.

  Liu X, Zhang J, Yuan C, Du G, Han S, Shi J, Sun J, Zhang B. Liu X, et al. Microb Cell Fact. 2023 Mar 16;22(1):53. doi: 10.1186/s12934-023-02052-y. Microb Cell Fact. 2023. PMID: 36922830 Free PMC article.
• Crystal structure and site-directed mutagenesis of 3-ketosteroid Δ1-dehydrogenase from Rhodococcus erythropolis SQ1 explain its catalytic mechanism.

  Rohman A, van Oosterwijk N, Thunnissen AM, Dijkstra BW. Rohman A, et al. J Biol Chem. 2013 Dec 6;288(49):35559-68. doi: 10.1074/jbc.M113.522771. Epub 2013 Oct 28. J Biol Chem. 2013. PMID: 24165124 Free PMC article.
• Site-directed mutagenesis under the direction of in silico protein docking modeling reveals the active site residues of 3-ketosteroid-Δ¹-dehydrogenase from Mycobacterium neoaurum.

  Qin N, Shen Y, Yang X, Su L, Tang R, Li W, Wang M. Qin N, et al. World J Microbiol Biotechnol. 2017 Jul;33(7):146. doi: 10.1007/s11274-017-2310-x. Epub 2017 Jun 20. World J Microbiol Biotechnol. 2017. PMID: 28634712
• Targeted disruption of the kstD gene encoding a 3-ketosteroid delta(1)-dehydrogenase isoenzyme of Rhodococcus erythropolis strain SQ1.

  van Der Geize R, Hessels GI, van Gerwen R, Vrijbloed JW, van Der Meijden P, Dijkhuizen L. van Der Geize R, et al. Appl Environ Microbiol. 2000 May;66(5):2029-36. doi: 10.1128/AEM.66.5.2029-2036.2000. Appl Environ Microbiol. 2000. PMID: 10788377 Free PMC article.
• Cloning, sequencing, and expression of the Pseudomonas testosteroni gene encoding 3-oxosteroid delta 1-dehydrogenase.

  Plesiat P, Grandguillot M, Harayama S, Vragar S, Michel-Briand Y. Plesiat P, et al. J Bacteriol. 1991 Nov;173(22):7219-27. doi: 10.1128/jb.173.22.7219-7227.1991. J Bacteriol. 1991. PMID: 1657885 Free PMC article.