Surface structure of yeast protoplasts
- PMID: 4867751
- PMCID: PMC252067
- DOI: 10.1128/jb.95.2.700-707.1968
Surface structure of yeast protoplasts
Abstract
The fine structure of the yeast cell wall during protoplast formation was studied by means of phase-contrast microscopy and the freeze-etching technique. The freeze-etching results indicated that at least in some cases the entire wall substance was not removed from the surface of the protoplasts. After a treatment of 30 min to 3 hr with 2% snail enzymes, an innermost thin wall layer as well as remnants of the fibrillar middle layer sometimes could be demonstrated.
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