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. 1968 Mar;95(3):1040-50.
doi: 10.1128/jb.95.3.1040-1050.1968.

Screening of Escherichia coli temperature-sensitive mutants by pretreatment with glucose starvation

D Boyd, R Nixon, S Gillespie, D Gillespie

Screening of Escherichia coli temperature-sensitive mutants by pretreatment with glucose starvation

D Boyd et al. J Bacteriol. 1968 Mar.

Abstract

A system for screening Escherichia coli temperature-sensitive mutants is described. The system involves glucose starvation and minimizes ambiguities introduced by the interdependencies of macromolecular synthesis during balanced growth. The system permits the quick recognition of protein synthesis mutants and their classification into two general catagories. Complete protein synthesis mutants are unable to make any polypeptide material, whereas partial protein synthesis mutants are able to produce inactive proteins. The phenotypes of several mutants are described.

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References

    1. C R Hebd Seances Acad Sci. 1962 Feb 19;254:1517-9 - PubMed
    1. J Mol Biol. 1961 Jun;3:318-56 - PubMed
    1. Mutat Res. 1966 Feb;3(1):13-24 - PubMed
    1. J Bacteriol. 1965 Mar;89:706-11 - PubMed
    1. Genetics. 1965 Jan;51:157-66 - PubMed

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