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. 1968 Mar;95(3):991-7.
doi: 10.1128/jb.95.3.991-997.1968.

Selection of temperature-sensitive activating enzyme mutants in Escherichia coli

S Kaplan, D Anderson

Selection of temperature-sensitive activating enzyme mutants in Escherichia coli

S Kaplan et al. J Bacteriol. 1968 Mar.

Abstract

A method based on temperature-conditional resistance to thymineless death has been designed to facilitate the isolation of amino acid-activating enzyme mutants. This method may be modified to obtain a greater or lesser proportion of activating enzyme mutants. In the latter instance, an increased proportion of temperature-sensitive macromolecule mutants of other types is obtained. Additional uses of this procedure are discussed.

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References

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