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. 1968 Nov 29;162(3857):1012-4.
doi: 10.1126/science.162.3857.1012.

Nonspecific staining: its control in immunofluorescence examination of soil

B B Bohlool, E L Schmidt

Nonspecific staining: its control in immunofluorescence examination of soil

B B Bohlool et al. Science. .

Abstract

Gelatin preparations were used to treat soil slides prior to addition of fluorescent antibody. Nonspecific staining was avoided, with no detectable interference to specific staining. Gelatin-rhodamine conjugates served to counterstain as well as to prevent nonspecific staining.

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