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. 1968 Nov;16(11):1640-5.
doi: 10.1128/am.16.11.1640-1645.1968.

Microtiter indirect hemagglutination procedure for identification of streptococcal M-protein antibodies

R A Zimmerman, J Mathews, E Wilson

Microtiter indirect hemagglutination procedure for identification of streptococcal M-protein antibodies

R A Zimmerman et al. Appl Microbiol. 1968 Nov.

Abstract

A number of investigators have attempted to utilize the hemagglutination system for detection of streptococcal type-specific antibody in human sera. Cross-reactions have made the procedure unreliable without cumbersome and time-consuming manipulation of the test sera. A method is described in which a microtiter indirect hemagglutination technique, using sensitized sheep erythrocytes, is sensitive, specific, and reliable for titration of type-specific antibody after naturally acquired or induced streptococcal infection.

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