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. 1979 Sep 15;35(9):1161-3.
doi: 10.1007/BF01963262.

Denaturation map of the ribosomal DNA of Lytechinus variegatus sperm

N K Mishra

Denaturation map of the ribosomal DNA of Lytechinus variegatus sperm

N K Mishra. Experientia. .

Abstract

Electron microscopy of the partially heat denatured ribosomal DNA (rDNA) from sea urchin (Lytechinus variegatus) sperm has demonstrated that it consists of repeating units of 3.6 +/- 0.2 micron, corresponding to a mol.wt of 7.2 +/- 0.4 x 10(6). Based on differential denaturability, each repeat unit is divided into 2 regions. The larger region of 2.47 +/- 0.11 micron (mol.wt 4.9 +/- 0.22 x 10(6)) corresponds in length to the ribosomal precursor RNA of sea urchins and the smaller, GC-rich, subunit of 1.16 +/- 0.09 micrometer (mol.wt 2.3 +/- 0.18 x 10(6)) is presumed to contain non-transcribed spacer sequences.

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