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. 1969 Jun;63(2):556-62.
doi: 10.1073/pnas.63.2.556.

Host specificity of DNA produced by Escherichia coli. XI. In vitro modification of phage fd replicative form

U Kühnlein, S Linn, W Arber

Host specificity of DNA produced by Escherichia coli. XI. In vitro modification of phage fd replicative form

U Kühnlein et al. Proc Natl Acad Sci U S A. 1969 Jun.

Abstract

An enzymatic activity, having the properties expected of a B-specific host-controlled modification enzyme, has been purified from an extract of Escherichia coli strain B. This activity renders the unmodified replicative form of phage fd resistant to B-specific restriction and is only present in strains carrying intact genes for type B modification. In phosphate buffer, the enzyme acts optimally at pH 6 and is dependent upon a single cofactor, S-adenosylmethionine.

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References

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