Chromosomal location of ribosomal protein cistrons determined by intergeneric bacterial mating

Abstract

Intergeneric mating between Escherichia coli and Salmonella typhosa was used to locate at least three 30S ribosomal proteins near the streptomycin locus in the region of 54 to 66 min of the E. coli map. This procedure utilizes differences in the electrophoretic patterns of 30S ribosomal protein of the parents. The results show that cistrons for 30S proteins of E. coli can replace those of S. typhosa in the Salmonella genome. Moreover, in a diploid hybrid with a Salmonella endogenote and an E. coli exogenote, both sets of cistrons are expressed.