Micro test for streptococcal anti-deoxyribonuclease B

Abstract

A methyl green decolorization micro test for the determination of streptococcal anti-deoxyribonuclease B titers in serum is described. The micro test was compared with an alcohol precipitation macro test on 112 human sera. The anti-deoxyribonuclease B titers agreed within one dilution step for 85.7% of the 112 sera examined by both techniques. Both tests were about equal in the detection of titers above the upper limits of normal. The micro test requires smaller amounts of reagents and fewer man-hours to perform than the macro test. This translates into lower cost per test and more tests per man-hour.