doi: 10.1073/pnas.63.1.86.
Isolation of adenyl cyclase from Escherichia coli
- PMID: 4897910
- PMCID: PMC534038
- DOI: 10.1073/pnas.63.1.86
Item in Clipboard
Isolation of adenyl cyclase from Escherichia coli
Proc Natl Acad Sci U S A.
1969 May.
Abstract
We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg(++) requirement and is inhibited by fluoride and inorganic pyrophosphate.
Similar articles
-
Adenyl cyclase of Escherichia coli.Biochem Biophys Res Commun. 1969 Jul 7;36(1):42-6. doi: 10.1016/0006-291x(69)90646-9. Biochem Biophys Res Commun. 1969. PMID: 4894362 No abstract available.
-
Solubilization of myocardial adenyl cyclase.Biochem Biophys Res Commun. 1970 Jan 6;38(1):86-92. doi: 10.1016/0006-291x(70)91087-9. Biochem Biophys Res Commun. 1970. PMID: 4313926 No abstract available.
-
Adenyl cyclase in cell-free extracts of Escherichia coli.Folia Microbiol (Praha). 1969;14(3):185-9. doi: 10.1007/BF02872776. Folia Microbiol (Praha). 1969. PMID: 4306671 No abstract available.
-
Preparation of thyroid plasma membranes containing a TSH-responsive adenyl cyclase.Biochem Biophys Res Commun. 1970 Jul 13;40(1):171-8. doi: 10.1016/0006-291x(70)91062-4. Biochem Biophys Res Commun. 1970. PMID: 4318584 No abstract available.
-
THE ACTION OF EPINEPHRINE AND THE ROLE OF THE ADENYL CYCLASE SYSTEM IN HORMONE ACTION.Recent Prog Horm Res. 1965;21:623-46. Recent Prog Horm Res. 1965. PMID: 14321068 Review. No abstract available.
Cited by
-
Glucose inhibition of adenylate cyclase in intact cells of Escherichia coli B.Proc Natl Acad Sci U S A. 1974 Jun;71(6):2324-8. doi: 10.1073/pnas.71.6.2324. Proc Natl Acad Sci U S A. 1974. PMID: 4366761 Free PMC article.
-
Anthrax toxin edema factor: a bacterial adenylate cyclase that increases cyclic AMP concentrations of eukaryotic cells.Proc Natl Acad Sci U S A. 1982 May;79(10):3162-6. doi: 10.1073/pnas.79.10.3162. Proc Natl Acad Sci U S A. 1982. PMID: 6285339 Free PMC article.
-
Regulation of cyclic AMP levels in Arthrobacter crystallopoietes and a morphogenetic mutant.J Bacteriol. 1978 Jun;134(3):1064-73. doi: 10.1128/jb.134.3.1064-1073.1978. J Bacteriol. 1978. PMID: 207674 Free PMC article.
-
[Cyclic nucleotides as intracellular transmitter substances in hormone actions].Klin Wochenschr. 1971 Oct 1;49(19):1049-58. doi: 10.1007/BF01732913. Klin Wochenschr. 1971. PMID: 4329904 German. No abstract available.
-
Phosphoenolpyruvate:carbohydrate phosphotransferase system of bacteria.Microbiol Rev. 1985 Sep;49(3):232-69. doi: 10.1128/mr.49.3.232-269.1985. Microbiol Rev. 1985. PMID: 3900671 Free PMC article. Review. No abstract available.
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
