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. 1969 May;63(1):86-92.
doi: 10.1073/pnas.63.1.86.

Isolation of adenyl cyclase from Escherichia coli

M Tao, F Lipmann

Isolation of adenyl cyclase from Escherichia coli

M Tao et al. Proc Natl Acad Sci U S A. 1969 May.

Abstract

We have found a soluble cyclase, using for assay radioactively marked ATP as precursor. The reaction product was isolated by thin-layer chromatography and identified by specific degradation. After homogenization, part of the activity remained in the particulate fraction but could be easily extracted. The cyclase was concentrated 100-fold by conventional methods. The enzyme has a Mg(++) requirement and is inhibited by fluoride and inorganic pyrophosphate.

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References

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    1. J Biol Chem. 1968 Oct 25;243(20):5420-7 - PubMed
    1. Biochem Biophys Res Commun. 1968 Mar 27;30(6):656-64 - PubMed
    1. Biochim Biophys Acta. 1967 Nov 21;149(1):1-11 - PubMed

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