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. 1979;29(1):71-7.
doi: 10.1016/0022-1759(79)90127-3.

Enzyme-linked immunosorbent microassay for quantification of specific antibodies to collagen type I, II, III

B Gosslau, H J Barrach

Enzyme-linked immunosorbent microassay for quantification of specific antibodies to collagen type I, II, III

B Gosslau et al. J Immunol Methods. 1979.

Abstract

An enzyme-liked immunosorbent assay (ELISA) is described for determination of antibodies to collagen type I, II, III in serum or other body fluids. Polystyrene microplates are coated with either collagen I, II or III, and samples of body fluids being tested are incubated in the plates. Antibodies which attach specifically to the collagen are detected with anti-IgG antibody conjugated with peroxidase. Bound peroxidase is quantitatively estimated by the colour reaction produced with the substrate 5-aminosalicylic acid.

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