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. 1969 Dec;100(3):1302-10.
doi: 10.1128/jb.100.3.1302-1310.1969.

Temperature-sensitive initiation of chromosome replication in a mutant of Escherichia coli

P L Kuempel

Temperature-sensitive initiation of chromosome replication in a mutant of Escherichia coli

P L Kuempel. J Bacteriol. 1969 Dec.

Abstract

The properties of Escherichia coli mutant D2-47LT indicate that it is temperature-sensitive for a protein required for the initiation of chromosome replication. The results of several different experiments are consistent with this hypothesis, and no support was found for the alternate hypotheses tested. Although the strain is usually unable to initiate replication at 42 C, some of the initiation proteins are apparently synthesized at the restrictive temperature. This can cause initiation on partially replicated, but not completed, chromosomes. It appears that the temperature-sensitive protein is required for initiation on completed chromosomes.

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References

    1. Bacteriol Rev. 1966 Mar;30(1):3-32 - PubMed
    1. Proc Natl Acad Sci U S A. 1965 Dec;54(6):1728-35 - PubMed
    1. Biochim Biophys Acta. 1966 Oct 24;129(1):104-15 - PubMed
    1. C R Acad Sci Hebd Seances Acad Sci D. 1967 Mar 13;264(11):1541-4 - PubMed
    1. J Mol Biol. 1967 May 28;26(1):107-23 - PubMed

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