Incorporation of 5-diazouracil-2-14C into ribonucleic acids of Escherichia coli during division inhibition

Abstract

Radioactive diazouracil (DZU-2-(14)C) was rapidly incorporated into acid-insoluble material of Escherichia coli B. Isolated ribonucleic acid contained essentially all of the incorporated label, and this was solubilized by ribonucleases but not by deoxyribonuclease. A maximum of 45 to 50% of added label was incorporated at division-inhibitory and subinhibitory concentrations. Incorporation levels and filament-forming capacity were concomitantly depressed by preincubation of DZU in various medium components. The lower levels of incorporation brought about by preincubation were apparently related to an inherent instability or high reactivity of the DZU. The limit on incorporation of fresh DZU suggests the presence of geometrical isomers in the original DZU. The nature of these isomers and the reactivity of DZU are discussed in relation to its use in future cell division studies.