Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Apr;102(1):118-23.
doi: 10.1128/jb.102.1.118-123.1970.

Pyrimidine pools and macromolecular composition of pyrimidine-limited Escherichia coli

P P Dennis, R K Herman

Pyrimidine pools and macromolecular composition of pyrimidine-limited Escherichia coli

P P Dennis et al. J Bacteriol. 1970 Apr.

Abstract

The growth rate of a pyrimidine-requiring strain was controlled by limiting the concentration of exogenous orotic acid. As the steady state, pyrimidine-limited growth rate was decreased, the intracellular pyrimidine pools and the total nucleic acid per unit mass of culture also decreased. The ratio of deoxyribonucleic acid to protein remained constant, whereas the ratio of ribonucleic acid to protein decreased 30% over a threefold variation in growth rate (50- to 150-min doubling times). The intracellular uridine triphosphate and cytosine triphosphate pools also decreased (although not coordinately), and the pyrimidine biosynthetic enzymes were derepressed. Cell size was unaffected by pyrimidine-mediated variation of the growth rate.

PubMed Disclaimer

References

    1. Proc Natl Acad Sci U S A. 1958 Dec 15;44(12):1191-6 - PubMed
    1. J Bacteriol. 1968 Nov;96(5):1519-27 - PubMed
    1. Proc Natl Acad Sci U S A. 1966 Oct;56(4):1233-40 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Feb;62(2):475-82 - PubMed
    1. J Biol Chem. 1967 Oct 25;242(20):4715-21 - PubMed

MeSH terms

LinkOut - more resources