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. 1970 Sep;67(1):99-106.
doi: 10.1073/pnas.67.1.99.

Peptide chain termination with mammalian release factor

J L Goldstein, A L Beaudet, C T Caskey

Peptide chain termination with mammalian release factor

J L Goldstein et al. Proc Natl Acad Sci U S A. 1970 Sep.

Abstract

We report a method for the in vitro study of peptide chain termination in mammals. A proteinaceous release factor has been isolated from rabbit reticulocyte extracts. This factor and a polyribonucleotide template containing the bases U and A stimulate the release of N-formylmethionine from mammalian [N-formylmethionyl-tRNA(f).ribosome] intermediates. Our studies suggest that UAA is a terminator codon for mammalian cells.

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References

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