doi: 10.1073/pnas.67.1.99.
Peptide chain termination with mammalian release factor
- PMID: 4917818
- PMCID: PMC283173
- DOI: 10.1073/pnas.67.1.99
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Peptide chain termination with mammalian release factor
Proc Natl Acad Sci U S A.
1970 Sep.
Abstract
We report a method for the in vitro study of peptide chain termination in mammals. A proteinaceous release factor has been isolated from rabbit reticulocyte extracts. This factor and a polyribonucleotide template containing the bases U and A stimulate the release of N-formylmethionine from mammalian [N-formylmethionyl-tRNA(f).ribosome] intermediates. Our studies suggest that UAA is a terminator codon for mammalian cells.
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