Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Jun;45(3):598-605.
doi: 10.1083/jcb.45.3.598.

Membrane splitting in freeze-ethching. Covalently bound ferritin as a membrane marker

P Pinto da Silva, D Branton

Membrane splitting in freeze-ethching. Covalently bound ferritin as a membrane marker

P Pinto da Silva et al. J Cell Biol. 1970 Jun.

Abstract

The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin. Ferritin molecules were never observed on fracture faces, thus indicating that fracture does not show membrane-surface detail. Subliming away the surrounding ice did expose the ferritin on the membrane surface. These results were consistent with the concept that membranes split during the fracture process of freeze-etching.

PubMed Disclaimer

References

    1. J Mol Biol. 1965 Dec;14(2):598-602 - PubMed
    1. Proc Natl Acad Sci U S A. 1966 May;55(5):1048-56 - PubMed
    1. Blood. 1967 May;29(5):780-9 - PubMed
    1. Science. 1967 Nov 3;158(3801):655-7 - PubMed
    1. Exp Cell Res. 1967 Sep;47(3):648-53 - PubMed