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. 1970 Sep;103(3):650-5.
doi: 10.1128/jb.103.3.650-655.1970.

Regulation of thymidine metabolism in Escherichia coli K-12: optimal conditions for the assay of 1,5-phosphodeoxyribomutase in ultrasonic extracts

R J Bonney, H Weinfeld

Regulation of thymidine metabolism in Escherichia coli K-12: optimal conditions for the assay of 1,5-phosphodeoxyribomutase in ultrasonic extracts

R J Bonney et al. J Bacteriol. 1970 Sep.

Abstract

Assay conditions have been established which allow maximum expression of phosphodeoxyribomutase activity in ultrasonic extracts of Escherichia coli K-12. The enzyme requires 2-mercaptoethanol, manganous ions, and glucose 1,6-diphosphate for optimal activity. When cells are grown in minimal medium with glycerol as the carbon source and supplemented with 10(-3)m thymidine, phosphodeoxyribomutase is induced three- to four-fold. Cell pellets may be frozen for 24 hr before sonic disruption without loss of enzyme activity. The assay is highly reproducible.

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