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. 1970 Aug;66(4):1027-35.
doi: 10.1073/pnas.66.4.1027.

Specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: its implications in repression

G W Hatfield, R O Burns

Specific binding of leucyl transfer RNA to an immature form of L-threonine deaminase: its implications in repression

G W Hatfield et al. Proc Natl Acad Sci U S A. 1970 Aug.

Abstract

The in vitro assembly of L-threonine deaminase from its constituent parts results in the formation of an inactive species of this enzyme which specifically and reversibly binds leucyl-tRNA. The native, catalytically active enzyme, which is derived from this species in the presence of maturation-inducing ligands, does not bind tRNA. The possible involvement of this protein-tRNA complex in the repression of the ilv (ADE) operon is discussed.

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