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. 1970 Aug;66(4):1220-7.
doi: 10.1073/pnas.66.4.1220.

Mapping of a human genetic regulator element by somatic cell genetic analysis

R J Klebe, T Chen, R H Ruddle

Mapping of a human genetic regulator element by somatic cell genetic analysis

R J Klebe et al. Proc Natl Acad Sci U S A. 1970 Aug.

Abstract

The ES-2 esterase is a kidney-associated esterase and is expressed in a cloned mouse renal adenocarcinoma cell line. Somatic cell hybridization of the mouse renal adenocarcinoma with either mouse or human fibroblasts leads to the marked reduction of ES-2 esterase titer. The extinction of ES-2 esterase activity in mousexhuman hybrids is reversible. Extinction correlates with the presence of the human C(10) chromosome in the somatic cell hybrids; whereas reexpression of the ES-2 esterase is observed in hybrids which have lost C(10). Thus, the proposed regulator element involved is probably structurally linked to C(10); however, linkage to a member of the D or G groups cannot be completely excluded.

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