Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1970 Aug;66(4):1260-6.
doi: 10.1073/pnas.66.4.1260.

In vitro transcription of a transfer RNA gene

V Daniel, S Sarid, J S Beckmann, U Z Littauer

In vitro transcription of a transfer RNA gene

V Daniel et al. Proc Natl Acad Sci U S A. 1970 Aug.

Abstract

The Escherichia coli tRNA(Tyr) gene carried by the varphi80psu(3) + transducing phage was transcribed in vitro by DNA-dependent RNA polymerase. The enzymatically synthesized tRNA(Tyr)-like polynucleotide chains were detected by competition with purified E. coli(32)P-tRNA(Tyr) for specific hybridization sites on varphi80psu(3) + DNA. Analysis by sucrose gradient centrifugation showed the tRNA(Tyr)-like chains to possess a heterogeneous distribution with respect to sedimentation coefficients, with a broad peak around 8 S. The presence of the termination factor rho during the transcription did not significantly reduce the average size of the in vitro synthesized tRNA(Tyr)-like chains.

PubMed Disclaimer

References

    1. J Biol Chem. 1964 Apr;239:1177-87 - PubMed
    1. J Mol Biol. 1966 Oct 28;21(1):115-27 - PubMed
    1. J Mol Biol. 1965 Dec;14(2):522-7 - PubMed
    1. J Mol Biol. 1967 Nov 14;29(3):457-71 - PubMed
    1. Proc Natl Acad Sci U S A. 1962 Jul 15;48:1199-203 - PubMed

LinkOut - more resources