doi: 10.1007/BF00778418.
DNA degradation in isolated rat liver nuclei
- PMID: 492162
- DOI: 10.1007/BF00778418
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DNA degradation in isolated rat liver nuclei
Mol Biol Rep.
.
Abstract
Purified rat liver nuclei were incubated at 4 degrees C. After different intervals of incubation aliquots of the nuclear suspension were taken and DNA was extracted by a common SDS-phenol-chloroform procedure. The fractionation of DNA by agar gel electrophoresis revealed large DNA fragments. It was shown that the well-known DNA degradation to monomers and its multiples is preceeded by an earlier breakdown of DNA into characteristic large fragments.
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