Elution of antibodies from biopsy tissue
- PMID: 4921714
- PMCID: PMC476851
- DOI: 10.1136/jcp.23.7.629
Elution of antibodies from biopsy tissue
Abstract
Cryostat sections proved to be sufficient material for acid elution of bound antibodies in vitro or in vivo. By single reconstitution of the pH the eluate may be studied for antibodies with the immunofluorescence technique. This micro-elution method may be of value for the study of kidney biopsy tissues.
Similar articles
-
Immunofluorescent staining of rat gastric parietal cells.J Clin Pathol. 1971 Oct;24(7):675. doi: 10.1136/jcp.24.7.675-b. J Clin Pathol. 1971. PMID: 4940808 Free PMC article. No abstract available.
-
Tissue antibodies in idiopathic autoimmune haemolytic anaemia.Clin Exp Immunol. 1971 May;8(5):741-8. Clin Exp Immunol. 1971. PMID: 4931855 Free PMC article.
-
Improved method for detection of anti-tissue antibody by indirect immunofluorescence using fixed tissue.J Clin Lab Immunol. 1980 Sep;4(2):119-24. J Clin Lab Immunol. 1980. PMID: 7003146
-
[Application of the immunofluorescente (fluorescent antibody technique) in clinical immunology.--II. Application and significance (author's transl)].Immun Infekt. 1976 Jun;4(3):126-40. Immun Infekt. 1976. PMID: 134971 Review. German.
-
Elution of kidney-bound antibody by pH-shift does not discriminate between antibodies differing in functional affinity for their ligand.Int Arch Allergy Appl Immunol. 1988;87(3):327-33. Int Arch Allergy Appl Immunol. 1988. PMID: 3060433 Review.
Cited by
-
Naturally occurring immune-complex glomerulonephritis in monkeys (Macaca irus). I. Light, immunofluorescence and electron microscopic studies.Am J Pathol. 1974 Jul;76(1):145-64. Am J Pathol. 1974. PMID: 4276331 Free PMC article.
-
Immunofluorescence microscopy of healthy skin from patients with systemic lupus erythematosus: more than just the lupus band.Ann Rheum Dis. 1992 Jun;51(6):720-5. doi: 10.1136/ard.51.6.720. Ann Rheum Dis. 1992. PMID: 1616353 Free PMC article.
-
Antikeratin autoantibodies in the amyloid deposits of lichen amyloidosus and macular amyloidosis.Arch Dermatol Res. 1989;281(6):377-82. doi: 10.1007/BF00455320. Arch Dermatol Res. 1989. PMID: 2480751
-
Renal lesions of subacute infective endocarditis.Br Med J. 1974 Apr 6;2(5909):11-4. doi: 10.1136/bmj.2.5909.11. Br Med J. 1974. PMID: 4595180 Free PMC article.
-
Immunofluorescence study of amyloid P-component patterns in human nephropathies.Histochemistry. 1982;74(3):347-54. doi: 10.1007/BF00493434. Histochemistry. 1982. PMID: 6179901
References
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources