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. 1970 Nov;120(2):255-61.
doi: 10.1042/bj1200255.

Molecular weight of Escherichia coli beta-galactosidase in concentrated solutions of guanidine hydrochloride

R P Erickson

Molecular weight of Escherichia coli beta-galactosidase in concentrated solutions of guanidine hydrochloride

R P Erickson. Biochem J. 1970 Nov.

Abstract

The molecular weight of Escherichia coli beta-galactosidase was determined in 6m- and 8m-guanidine hydrochloride by meniscus-depletion sedimentation equilibrium, sedimentation velocity and viscosity. Sedimentation equilibrium revealed heterogeneity with the smallest component having a molecular weight of about 50000. At lower speeds, the apparent weight-average molecular weight is about 80000. By use of a calculation based on an empirical correlation for proteins that are random coils in 6m-guanidine hydrochloride, sedimentation velocity gave a molecular weight of 91000, and the intrinsic viscosity indicated a viscosity-average molecular weight of 84000. Heating in 6m-guanidine hydrochloride lowered the viscosity of beta-galactosidase in a variable manner.

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