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. 1971 Jan;105(1):190-9.
doi: 10.1128/jb.105.1.190-199.1971.

Effects of essential amino acid starvation in Streptococcus faecalis: structural change in the 50S ribosomal subunit

R J Ziegler, L Daneo-Moore

Effects of essential amino acid starvation in Streptococcus faecalis: structural change in the 50S ribosomal subunit

R J Ziegler et al. J Bacteriol. 1971 Jan.

Abstract

The 50S ribosomal subunits obtained from exponential-phase and valine-limited cultures of Streptococcus faecalis 9790 differed in protein content. Approximately 35% of the valine-limited subunits showed protein losses when analyzed by CsCl density centrifugation after formaldehyde fixation. Results of density gradient analysis and of acrylamide gel electrophoresis localized the protein to a "split" subunit fraction. No differences could be detected in the protein contents of 30S subunits from exponential-phase and valine-limited cultures.

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