doi: 10.1073/pnas.68.4.761.
Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II
- PMID: 4927672
- PMCID: PMC389037
- DOI: 10.1073/pnas.68.4.761
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Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II
Proc Natl Acad Sci U S A.
1971 Apr.
Abstract
The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.
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