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. 1971 Apr;68(4):761-4.
doi: 10.1073/pnas.68.4.761.

Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II

T Kornberg, M L Gefter

Purification and DNA synthesis in cell-free extracts: properties of DNA polymerase II

T Kornberg et al. Proc Natl Acad Sci U S A. 1971 Apr.

Abstract

The major DNA-synthesizing enzyme present in Pol A(1) (-)Escherichia coli (DNA polymerase II) has been purified to homogeneity as judged by polyacrylamide gel electrophoresis. The enzyme requires all four deoxynucleoside triphosphates, Mg(++), NH(4) (+), and native DNA for maximal activity. The enzyme activity is sensitive to sulfhydryl reagents and is insensitive to anti-DNA polymerase I antiserum. A second DNA-synthesizing enzyme, present in low amounts, has been identified in Pol A(1) (-) extracts. The relationship of this enzyme to DNA polymerases I and II is discussed.

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References

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    1. Nature. 1970 Oct 17;228(5268):223-6 - PubMed
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