Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Apr;68(4):824-7.
doi: 10.1073/pnas.68.4.824.

Genetic recombination in Escherichia coli: the role of exonuclease I

S R Kushner, H Nagaishi, A Templin, A J Clark

Genetic recombination in Escherichia coli: the role of exonuclease I

S R Kushner et al. Proc Natl Acad Sci U S A. 1971 Apr.

Abstract

The indirect suppression of recB(-) and recB(-)recC(-) mutations by the sbcB(-) allele is caused by the loss of a nuclease active on denatured DNA. Results from enzyme purifications and studies with a specific antiserum demonstrate that the activity present in sbcB(+) strains, and lost in sbcB(-) strains, is exonuclease I. It is likely that sbcB is the structural gene for exonuclease I. The loss of exonuclease I activity restores the recombination proficiency of Escherichia coli cells that has been lost by mutations in the recB and/or recC genes. This indicates that in the absence of the recB-recC-determined enzyme, exonuclease I prevents recombination. Hypothetical pathways illustrating this conclusion are presented.

PubMed Disclaimer

References

    1. J Mol Biol. 1966 Aug;19(2):442-54 - PubMed
    1. Bacteriol Rev. 1967 Dec;31(4):332-53 - PubMed
    1. J Bacteriol. 1969 Jan;97(1):244-9 - PubMed
    1. Genetics. 1968 Dec;60(4):641-60 - PubMed
    1. J Bacteriol. 1969 Nov;100(2):923-34 - PubMed

LinkOut - more resources