Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 May;68(5):964-8.
doi: 10.1073/pnas.68.5.964.

Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo

C M Bowman, J E Dahlberg, T Ikemura, J Konisky, M Nomura

Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo

C M Bowman et al. Proc Natl Acad Sci U S A. 1971 May.

Abstract

Treatment of sensitive Escherichia coli cells with colicin E3 leads to inactivation of 30S ribosomal subunits. In vitro reconstitution of 30S subunits indicates that the E3-induced defect lies solely in the 16S RNA. 16S RNA from E3-treated cells lacks several T(1) RNase oligonucleotides of normal 16S RNA, including the one from the 3'-end of the 16S RNA, as analyzed by the fingerprint technique of Sanger. An RNA fragment about 50 nucleotides long has been isolated from E3-treated cells. This RNA contains the original 3'-terminal oligonucleotide and other oligonucleotides missing in the E3-16S RNA. The results show that colicin E3 treatment causes the cleavage of 16S RNA at a specific position near the 3'-terminus.

PubMed Disclaimer

References

    1. J Mol Biol. 1965 Sep;13(2):373-98 - PubMed
    1. J Bacteriol. 1966 Feb;91(2):685-94 - PubMed
    1. J Mol Biol. 1967 Feb 14;23(3):337-53 - PubMed
    1. J Mol Biol. 1967 Jun 14;26(2):181-95 - PubMed
    1. Annu Rev Microbiol. 1967;21:257-84 - PubMed

LinkOut - more resources