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. 1971 Apr;21(4):585-7.
doi: 10.1128/am.21.4.585-587.1971.

Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity

R V Lachica, C Genigeorgis, P D Hoeprich

Metachromatic agar-diffusion methods for detecting staphylococcal nuclease activity

R V Lachica et al. Appl Microbiol. 1971 Apr.

Abstract

Based on the metachromatic property of Toluidine Blue O, three, convenient agar-diffusion methods have been developed that enable detection of the nuclease of Staphylococcus aureus at concentrations as low as 0.005 mug/ml in agar and broth cultures. The interactions of agar and deoxyribonucleic acid with Toluidine Blue O are discussed.

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References

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