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. 1979 Aug 10;6(11):3535-42.
doi: 10.1093/nar/6.11.3535.

Selective staining by 4', 6-diamidine-2-phenylindole of nanogram quantities of DNA in the presence of RNA on gels

J Kapuściński, K Yanagi
Free PMC article

Selective staining by 4', 6-diamidine-2-phenylindole of nanogram quantities of DNA in the presence of RNA on gels

J Kapuściński et al. Nucleic Acids Res. .
Free PMC article

Abstract

4', 6-Diamidine-2-phenylindole.2HCl (DAPI) forms fluorescent complexes with double-stranded (ds) DNA but not with ds RNA as shown by fluorescence titration. The widely used dye ethidium bromide (EB) forms fluorescent complexes with both types of nucleic acids. Also, in contrast to EB, DAPI forms much weaker fluorescent complexes with single-stranded DNA than with ds DNA. These observations were utilized to develop staining procedures for the selective visualization of ds DNA on gels. The use of DAPI in addition to EB for staining makes possible the localization of ds DNA and other species of nucleic acids on a single gel.

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References

    1. Biochemistry. 1978 Feb 21;17(4):601-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Nov;74(11):4835-8 - PubMed
    1. Mol Cell Biochem. 1977 Dec 29;18(2-3):81-6 - PubMed
    1. Nucleic Acids Res. 1978 Oct;5(10):3775-99 - PubMed
    1. Anal Biochem. 1977 Nov;83(1):252-7 - PubMed