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. 1979 Aug 24;6(12):3773-84.
doi: 10.1093/nar/6.12.3773.

Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts

P F Lurquin
Free PMC article

Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts

P F Lurquin. Nucleic Acids Res. .
Free PMC article

Abstract

Lecithin and lecithin/cholesterol liposomes formed in aqueous solutions of DNA entrap covalently closed circular, open circular and linear DNA molecules of size up to at least 13 kilobases. The sequestered DNA molecules are efficiently protected against exogenous deoxyribonuclease action although nicking and linearization of circular DNA can be observed. The size of these liposomes ranges from approximately 0.5 to 7.5 mu with an average of 2.5--4 mu. DNA filled liposomes strongly interact with plant protoplasts under conditions inducing protoplast fusion. Results suggest that sequestered plasmid DNA can be transferred to protoplast nuclei.

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