Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1979 Aug 24;6(12):3773-84.
doi: 10.1093/nar/6.12.3773.

Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts

P F Lurquin
Free PMC article

Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts

P F Lurquin. Nucleic Acids Res. .
Free PMC article

Abstract

Lecithin and lecithin/cholesterol liposomes formed in aqueous solutions of DNA entrap covalently closed circular, open circular and linear DNA molecules of size up to at least 13 kilobases. The sequestered DNA molecules are efficiently protected against exogenous deoxyribonuclease action although nicking and linearization of circular DNA can be observed. The size of these liposomes ranges from approximately 0.5 to 7.5 mu with an average of 2.5--4 mu. DNA filled liposomes strongly interact with plant protoplasts under conditions inducing protoplast fusion. Results suggest that sequestered plasmid DNA can be transferred to protoplast nuclei.

PubMed Disclaimer

References

    1. Annu Rev Genet. 1977;11:79-101 - PubMed
    1. FEBS Lett. 1977 Jul 1;79(1):80-4 - PubMed
    1. Mol Gen Genet. 1977 Jul 20;154(2):113-21 - PubMed
    1. Prog Nucleic Acid Res Mol Biol. 1977;20:161-207 - PubMed
    1. FEBS Lett. 1978 Mar 15;87(2):277-82 - PubMed