Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 1971 Jul;68(7):1512-5.
doi: 10.1073/pnas.68.7.1512.

Acetyl CoA carboxylase: isolation and characterization of native biotin carboxyl carrier protein

R R Fall, A M Nervi, A W Alberts, P R Vagelos

Acetyl CoA carboxylase: isolation and characterization of native biotin carboxyl carrier protein

R R Fall et al. Proc Natl Acad Sci U S A. 1971 Jul.

Abstract

A large form of biotin carboxyl carrier protein (BCCP(L)) has been isolated from extracts of Escherichia coli. It has a minimal molecular weight of 20,000, according to its behavior on sodium dodecylsulfate-polyacrylamide gel electrophoresis, and contains approximately 1 mol of biotin per 22,000 g of protein. BCCP(L) exhibits K(m) values, in the biotin carboxylase and transcarboxylase half-reactions of acetyl CoA carboxylase, of 2 x 10(-7) M and 4 x 10(-7) M, respectively; these values are 50-100 times lower than those obtained with smaller forms of BCCP previously isolated. Electrophoresis of crude extracts of E. coli indicates that the major biotin-containing protein migrates at the same rate as BCCP(L), which suggests that BCCP(L) is the native form of BCCP in E. coli.

PubMed Disclaimer

References

    1. Anal Biochem. 1967 Jul;20(1):150-4 - PubMed
    1. Proc Natl Acad Sci U S A. 1968 Feb;59(2):561-8 - PubMed
    1. J Biol Chem. 1969 Aug 25;244(16):4406-12 - PubMed
    1. J Biol Chem. 1969 Sep 25;244(18):5074-80 - PubMed
    1. Proc Natl Acad Sci U S A. 1969 Aug;63(4):1319-26 - PubMed

LinkOut - more resources