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. 1971 Oct;108(1):300-8.
doi: 10.1128/jb.108.1.300-308.1971.

Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells

S B Levy

Physical and functional characteristics of R-factor deoxyribonucleic acid segregated into Escherichia coli minicells

S B Levy. J Bacteriol. 1971 Oct.

Abstract

Both fi(+) and fi(-) type R factors function as intact replicative units when segregated into Escherichia coli minicells. Hybridization studies demonstrate that at least 95% of the deoxyribonucleic acid (DNA) in R(+) minicells is episomal in origin. About half of the DNA can be extracted in a closed circular form and about 75% of the DNA is membrane associated. DNA, ribonucleic acid, and protein synthesis proceeds in R(+) minicells in contrast to R(-) minicell controls. The system offers a unique opportunity to study a relatively small replicative unit in a native cell environment and a simple means of isolating large quantities of episomal DNA.

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