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. 1971 Jun;123(2):163-70.
doi: 10.1042/bj1230163.

The active centre of rabbit muscle triose phosphate isomerase. The site that is labelled by glycidol phosphate

J C Miller, S G Waley

The active centre of rabbit muscle triose phosphate isomerase. The site that is labelled by glycidol phosphate

J C Miller et al. Biochem J. 1971 Jun.

Abstract

1. Glycidol (2,3-epoxypropanol) phosphate is a specific irreversible inhibitor of rabbit muscle triose phosphate isomerase (EC 5.3.1.1); the site of attachment has now been studied. 2. The labelled enzyme was digested with pepsin and a modified peptide isolated. The sequence of the peptide is: Ala-Tyr-Glu-Pro-Val-Trp. 3. It is the glutamic acid residue in this peptide that is labelled: the peptide is thus a gamma-glutamyl ester derived from glycerol phosphoric acid. The same site is labelled by a mixture of glycidol and inorganic phosphate. 4. Kinetic and stereochemical features of these reactions are discussed.

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