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. 1971 Nov;108(2):690-4.
doi: 10.1128/jb.108.2.690-694.1971.

Biochemical characterization of the ctr mutants of Escherichia coli

H G Morse, W K Penberthy, M L Morse

Biochemical characterization of the ctr mutants of Escherichia coli

H G Morse et al. J Bacteriol. 1971 Nov.

Abstract

A test procedure based on complementation in mixed extracts is described for the assay of heat-stable protein and enzyme I of the phosphoenolpyruvate-dependent phosphotransferase system. The test was used to assay a collection of pleiotropic carbohydrate mutants of Escherichia coli (ctr mutants) and revertants of these mutants. All mutants were found to lack enzyme I of the phosphoenolpyruvate-dependent transferase system. Revertants of these mutants to complete wild phenotype regained enzyme I-forming ability. Reversion to partial wild type was not accompanied by restoration of enzyme I-forming ability.

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References

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